Figure 5.

Quantitative RT-PCR analysis of miR-34a expression levels in 24 paired gastric carcinoma and adjacent normal tissues. Left curve: Each column indicates the fold change of expression value (2^−∆∆Ct) of the genes in individual tumor (black column) or control (gray column) tissue normalized by the median expression levels (∆Ct) of the all control tissues, as described in “Methods” section. Numbers on the bottom axis correspond to case numbers in Table 1. Right curve: Overall RNA expression levels in tumor samples compared to non-tumor samples. Quantitative RT-PCR data were presented as box plots showing the median of overall RNA expression in tumor samples relative to non-tumor samples. The lines inside the boxes denote the medians. The whiskers of box plots: 10–90%. Decreased levels of miR-34a in the tumor tissues were not statistically significant (median FC = 0.915). Statistical differences were evaluated between two groups by non-parametric Mann–Whitney test.