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. 2020 Jul 23;10:12342. doi: 10.1038/s41598-020-69308-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative RT-PCR analysis of miR-181a expression levels in 24 paired gastric carcinoma and adjacent normal tissues. Left curve: Each column indicates the fold change of expression value (2^−∆∆Ct) of the genes in individual tumor (black column) or control (gray column) tissue normalized by the median expression levels (∆Ct) of the all control tissues, as described in “Methods” section. Numbers on the bottom axis correspond to case numbers in Table 1. Right curve: Overall RNA expression levels in tumor samples compared to non-tumor samples. Quantitative RT-PCR data were presented as box plots showing the median of overall RNA expression in tumor samples relative to non-tumor samples. The lines inside the boxes denote the medians. The whiskers of box plots: 10–90%. Increased level of miR-181a in the tumor tissues were not statistically significant (median FC = 1.5). Statistical differences were evaluated between two groups by non-parametric Mann–Whitney test.