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. 2020 Jul 21;9:e56038. doi: 10.7554/eLife.56038

Figure 4. Evolutionary arms races between host and parasitic RNAs.

(A) Competitive replication assays of each pair of the evolved host and parasitic RNA clones. The RNA replication reactions were performed with 10 nM of the host and parasitic RNAs for 3 hr, and each concentration was measured by sequence-specific RT-qPCR. Error bars represent standard errors of three independent competition assays. (B) Schematic representation of the host-parasite relationships among the RNA clones.

Figure 4—source data 1. Dominant mutations in Host-99 and Host-115.
Host-99 and Host-115, which exhibited distinct parasite resistance (Figure 4), have very different mutation sets with only three redundant dominant mutations each other. Mutation indexes correspond to those in Figure 2—figure supplement 3.

Figure 4.

Figure 4—figure supplement 1. Combinatorial competitive replication assay of Hosts-99 and −115 with Parasites-β99 and -γ115.

Figure 4—figure supplement 1.

The RNA replication reactions were performed with 10 nM of the host and parasitic RNAs for 3 hr and each concentration was measured by sequence-specific RT-qPCR. The three results (Host-99 vs parasite-β99, Host-115 vs parasite-β99, and Host-115 vs parasite-γ115) are the same as those in Figure 4. Error bars represent standard errors of three independent competition assays.