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Generation of hiPSCs-derived hepatic organoids. (A) Schematic diagram of the generation protocol from hiPSCs to hepatic organoid. (B) Representative bright field image of hiPSCs, definitive endoderm, hepatic endoderm, and hepatic organoids. (C) Bright field image of organoids immediately after Matrigel embedding (D0) and in the same field during culture (D1 to D3). (D) Representative immunofluorescence images of the hepatic organoids stained with E-cadherin and ALB.
