Fig. 4.

Differentiation potential of the long-term expanded hepatic organoids. (A) Schematic diagram of organoid differentiation for further hepatic maturation. Hepatic medium (HM); Expansion medium (EM); and Differentiation medium (DM). (B) mRNA expression levels of ALB, RBP4, and CYP3A4 in HM- or DM-cultured organoids at passage 10. (C) Representative morphology and (D) mRNA expression levels of ALB, RBP4, and CYP3A4 of differentiated hepatic organoids at each indicated passage. Data are the mean±SEM (n=3) and analyzed by Student’s t-test. *p<0.05; **p<0.01; and ***p<0.001.