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. 2020 Jun 28;11(17):5106–5117. doi: 10.7150/jca.45822

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Down-regulation of MST4 in hepatocellular carcinoma (HCC). (A) MST4 mRNA expression in the indicated 7 HCC cell lines and normal human hepatic cell line LO2 detected by qRT-PCR. (B) MST4 mRNA expression in HCC and matched NT specimens from 66 HCC patients detected by qRT-PCR (P value shown was calculated by a paired Student's t test). (C) Representative images of MST4 protein localization in Bel-7402 cells by immunofluorescence (IF) staining. (D) Representative images showing MST4 protein weak expression in HCC and high expression in NT specimens examined by immunohistochemistry (IHC) staining. (E) The percentage of different MST4 IHC staining levels in the HCC and NT specimens. NT: adjacent non-tumor liver tissue.