Figure 2.

Effects of Ca²⁺ free solution and SAR7334 on [Ca²⁺]_r and [Na⁺]_r in hippocampal pyramidal neurons. Panel (A) shows that removal of extracellular Ca²⁺ significantly lowered the [Ca²⁺]_r in all age-group; however, the magnitude of the reduction of [Ca²⁺]_r was more evident in aged than young neurons. Panels (B,C) illustrate SAR7334 elicited a dose-dependent reduction of [Ca²⁺]_r in the young, middle-aged and aged neurons, being more marked in aged neurons. Panel (D) exemplifies the reduction provoked by SAR7334 on [Na⁺]_r, which was greater in aged than young neurons. On the horizontal axis are indicated the experimental conditions used to measure [Ca²⁺]_r and [Na⁺]_r. For [Ca²⁺]_i measurements: n_mice = 3–5/age group, n_cell = 9–16/age group. For [Na⁺]_i measurements: n_mice = 4/per age group, n_cell = 12–15/age group. Values are expressed as means ± S.D. for each condition. One-way ANOVA followed by Tukey’s post hoc comparisons, *p ≤ 0.05.