Fig. 4.

Effect of antibody treatment on the expression of the Ki-67 proliferation marker. All experiments related to this figure were performed on mice aged 6 to 8 weeks (see Materials and Methods, section 2.7). a, Representative transversal sections of mouse dorsal skin showing cells that line the envelope of sebaceous glands labeled with an antibody that binds Ki-67 proteins (green). Shown are maximal projection renderings of nine consecutive confocal optical sections taken at 1.0 μm intervals; scale bar: 30 µm. b, Ki-67 immunoreactivity data (mean ± s.e.m.) obtained by analyzing 7 microscopic fields of view, each field=323 × 323 µm², from n=3 non-treated mice (wt and mutant littermates) and n=2 treated mice (mutant + abEC1.1 cream, or mutant + isotype antibody cream); P = p-value (ANOVA).