Fig. 3. Effect of curcumin on acrolein-induced intracellular peroxide production.

(A) Cells were incubated with (black area) or without (white area) 10 µM acrolein for 30 minutes, and then treated with a peroxide sensitive dye, H₂DCF-DA (20 µM) during the final 30 minutes of each treatment. The relative peroxide concentrations in cells were then measured by flow cytometry. (B) After preincubation with 10 mM NAC for 4 hours, the cells were treated with (black area) or without (white area) 10 µM acrolein and flow cytometric analysis was performed. X-axis represents intensity of fluorescence.

NAC, N-acetyl-cysteine.