Skip to main content
. 2020 Jul 17;8:818. doi: 10.3389/fbioe.2020.00818

FIGURE 1.

FIGURE 1

Construction of a synthetic promoter library in S. erythraea. (A), four sets of templates for the construction of a SPL. -35 or -10 region is in red or blue, respectively. Ribosome binding site (RBS) is underlined. The degenerate nucleotides: N = A/C/G/T, W = A/T, V = A/C/G, D = A/G/T, H = A/C/T, S = C/G. (B), characterization of promoter strength shown as the total GusA activity per gram of dry cell weight (DCW). Asterisks above bars indicate that these promoters would be used to drive the expression of NOX.