TABLE 1.
Strains and plasmids used in this study.
| Designation | Genotype or description | References |
| S. erythraea strains | ||
| E3 | Industrial erythromycin producing strain | Our lab |
| E3:GUSA | Single copy of ermE*p-gusA expressed in E3 | This work |
| D1N | Single copy of D1W12p-nox expressed in E3 | This work |
| A2N | Single copy of 2A23p-nox expressed in E3 | This work |
| R3N | Single copy of ermE*p-nox expressed in E3 | This work |
| E3H | F1F0-ATPase overexpressed in E3, promoted by its native promoter, lower [ATP]/[ADP] ratio compared to E3 | Li et al., 2020 |
| E. coli strains | ||
| Top 10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA1 araΔ139Δ(ara-leu)7697 galU galKrpsL (StrR) endA1 nupG, used for plasmid constructions | |
| ET12567 | F- dam-13:Tn9 dcm-6 hsdM hsdR zjj-202:Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44, used for conjugation | |
| Plasmids | ||
| pIB139 | An integrative plasmid containing oriT, attP, int, aac(3)IV and ermE*p | Wilkinson et al., 2002 |
| pSETGUS | gusA containing BamHI fragment cloned into BamHI site of pSET152 | Myronovskyi et al., 2011 |
| pIBGUS | pIB139 derivative with ermE*p in front of gusA | This work |
| pSPLGUS | pIB139 derivatives with synthetic promoters in front of gusA | This work |
| pNOX | CDS of nox inserted into pIB139 without any promoters | This work |
| pD12nox | D1W12p-nox inserted into pIB139 without original ermE*p | This work |
| p2A23nox | 2A23p-nox inserted into pIB139 without original ermE*p | This work |
| pIBnox | CDS of nox inserted into pIB139, promoted by ermE*p | This work |