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. 2020 Jul 17;8:632. doi: 10.3389/fcell.2020.00632

FIGURE 2.

FIGURE 2

Let-7g* and miR-98 selectively target different cytokine mRNAs. Prediction analysis for miR-98 and let-7g* for the ability to create miR-mRNA hybrid with IP-10 (A) and CXCL1 (D) 3′ UTRs, with minimum free energy recorded. Luciferase activity for IP-10′s 3′ UTR reporter in HEK-293 cells transfected with mimic oligos of miR-98 (B) or let-7g* (C). Luciferase activity for CXCL1’s 3′ UTR reporter with mimic oligos of miR-98 (E) or let-7g* (F). 3′ UTR sequences of IP-10 and CXCL1 were mutated in the seed-binding site (underlined and bolded). Data are shown as mean ± SD. *p < 0.05.