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. 2020 Jul 17;11:1586. doi: 10.3389/fmicb.2020.01586

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Copyright © 2020 Gómez, Alvarez, Molina, Soto-Shara, Daza-Castro, Flores, León and Oñate.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modeling and purification of Zn-dependent metalloproteinase. (A) ProSA-web Z-score plot for 3D structure of the hypothetical protein after refinement. The Z-score of the final model is −4.52. The black dot represents the hypothetical protein. (B) 3D structure according to the I-TASSER server after being refined and visualized by Chimera PyMOL. (C) Purification of the ZnMP codifying for ORF BAB1_0270 of B. abortus 2308. ZnMP sequences were cloned in the expression vector pColdII (pColdII-ZnMP), and the recombinant vector was used to transform the Escherichia coli BL21 (DE3) strain. Western blot was performed to visualized protein using anti-6x-HisTag antibodies. MW: molecular weight marker in kDa; 1: recombinant protein purified from E. coli.