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. 2020 Jul 17;11:1586. doi: 10.3389/fmicb.2020.01586

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Copyright © 2020 Gómez, Alvarez, Molina, Soto-Shara, Daza-Castro, Flores, León and Oñate.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A Zinc-dependent metalloproteinase of B. abortus is an operon that forms a putative type II toxin-antitoxin. Identification of the transcriptional unit (operon) constituted by ORF BAB1_0270 and a transcriptional regulator in B. abortus 2308 expressed in (A) the genomic DNA and (B) the cDNA from total RNA. MW: Molecular weight; lane 1: Transcriptional regulator (357 bp); lane 2: BAB1_0270 (549 bp); and lane 3: operon constituted by ORF BAB1_0270-transcriptional regulator (906 bp). (C) Hypothetical Type II toxin-antitoxin (TA) model for operon constitute by ZnMP and transcriptional regulator. Toxin (ZnMP) and anti-toxin (transcriptional regulator) are transcribed to mRNA together. Proteases are activated under stress conditions, cleaving the anti-toxin, which increases the levels of toxin-free, inducing various biological functions in bacteria. Predicted promoter at site -35 and -10 binding by RNA polymerase sigma factor rpoD. ATG A: nucleotides shared between final part of transcriptional factor and metalloproteinase codified for ORF BAB1_0270.