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. 2020 Jul 17;11:1586. doi: 10.3389/fmicb.2020.01586

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Copyright © 2020 Gómez, Alvarez, Molina, Soto-Shara, Daza-Castro, Flores, León and Oñate.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Brucella strains growth and gene expression under acidic stress. (A) Growth curves of B. abortus 2308, B. abortus Δ270, and B. abortus Δ270C strains cultured in acidic medium (pH 5.5). (B) Linear regression analysis of pH changes in the medium during the growth of Brucella strains. (C) The relative expression of vjbR, hutC, bvrR, virB1, virb2, virB5, btpA, vceA, and vceC induced in B. abortus 2308 (wt) and B. abortus Δ270 cultured in medium with pH 5.5. The gene expression was calculated by 2^−ΔΔCT method using qPCR assays at 24 h. The housekeeping genes gyrA and 16s were used as reference genes. Results were expressed as the mean ± standard deviation. P value < 0.05 were considered statistically significant, where * denotes values of P < 0.05 and **** denotes values of P < 0.0001. All assays were performed in triplicate.