. Author manuscript; available in PMC: 2020 Jul 24.

Published in final edited form as: J Proteome Res. 2016 Aug 23;15(9):2891–2899. doi: 10.1021/acs.jproteome.5b00996

Table 1.

DownPeptide Reading Frames

yeast	Frame 1	Frame 2	Frame 3
detected downPeptides (OMSSA)	0.52^a	0.40	0.08
detected downPeptides (Mascot)	0.46^a	0.50	0.04
all downAUGs within 100 nt of annAUG	0.34	0.54	0.12
Drosophila	Frame 1	Frame 2	Frame 3

detected downPeptides	0.73^a	0.20	0.06
all downAUGs within 100 nt of annAUG	0.44	0.43	0.13

Chi-square tests indicated that frame-1 downPeptide frequencies are significantly elevated compared to all downAUGs within 100 nt of the annAUG and with downORF > 12 nt (yeast Mascot: p = 0.014, 5% FDR; yeast OMSSA: p = 3.10 × 10⁻⁵, 5% FDR; Drosophila: p = 2.15 × 10⁻²⁵, 2% FDR).