FIGURE 2.

Function of enT3SS in non-native host, E. coli. (A) The secreted SptP was detected in the culture supernatant of E. coli containing plasmids for enT3SS and the reporter plasmid encoding the Salmonella effector protein, SptP. (B) A map of pSL002 encoding MTD with 167 amino acids of SptP at the N-terminus (left panel) and the representative western blot analysis of the secreted MTD in culture supernatant (right panel) (n = 3). The pellet fraction from each sample was also tested for the comparison of expression level (middle blot; pellet). WT indicates the results from MG1655 carrying only pSL002. enT3SS was induced by the addition of 10 μM of IPTG and AHL, respectively.