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. 2020 Jul 17;10:363. doi: 10.3389/fcimb.2020.00363

Figure 3.

Figure 3

Intracellular NAD+/NADH elevated due to heat-stress, nuclear protein from heat shock parasites binds specifically to ERM and overexpression of ERM-BP makes parasites more resistant to heat stress. (A) Measurement of intracellular NAD+/NADH in heat-shock and untreated E. histolytica. Data are mean ±s.d. (n = 3) Student's t-test; **p < 0.01. (B) EMSA was performed using crude nuclear extracts from control and heat-stressed E. histolytica cells. EMSA results are shown in the presence and absence of different components marked as “+” and “–,” respectively. Radiolabeled ERM probe was used in each reaction. Unlabeled ERM probe at 10× and 100× was used as a specific competitor as indicated. The arrow indicates the major specific band in the gel shift assay; free probe is at the bottom. (C) Viability assay of control and overexpressed-ERM-BP cells from both E. histolytica and E. invadens were performed by FDA hydrolysis assay. Data are mean ± s.d. (n = 2) Student's t-test; *P < 0.05.