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. 2018 Feb 13;26(4):671–682. doi: 10.1111/1744-7917.12566

Table 2.

Details of primers used in this study

Target gene Primer sequences (5′→3′) Amplicon size Annealing temperature Reference
gatB

  • Forward: GAKTTAAAYCGYGCAGGBGTT

  • Reverse: TGGYAAYTCRGGYAAAGATGA

 471 bp 54 °C Baldo et al., 2006
coxA

  • Forward: TTGGRGCRATYAACTTTATAG

  • Reverse: CTAAAGACTTTKACRCCAGT

 487 bp 54 °C Baldo et al., 2006
hcpA

  • Forward: GAAATARCAGTTGCTGCAAA

  • Reverse: GAAAGTYRAGCAAGYTCTG

 515 bp 54 °C Baldo et al., 2006
ftsZ

  • Forward: ATYATGGARCATATAAARGATAG

  • Reverse: TCRAGYAATGGATTRGATAT

 524 bp 52 °C Baldo et al., 2006
fbpA

  • Forward: GCTGCTCCRCTTGGYWTGAT

  • Reverse: CCRCCAGARAAAAYYACTATTC

 509 bp 58 °C Baldo et al., 2006
wsp

  • Forward: GTCCAATARSTGATGARGAAAC

  • Reverse: CYGCACCAAYAGYRCTRTAAA

 603 bp 58 °C Baldo et al., 2006
D. citri’s mtCOI

  • Forward: AGGAGGTGGAGACCCAATCT

  • Reverse: TCAATTGGGGGAGAGTTTTG

 821 bp  53 °C  Boykin et al., 2012