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. 2018 Nov 1;1438(1):3–17. doi: 10.1111/nyas.13976

Figure 4.

Figure 4

PKR promotes IFN‐β production in response to SPPV infection in HeLa cells. (A) HeLa cells were treated with poly (I:C) (10 μg/mL) or infected with SPPV Gulang/2009 at an MOI of 3 for 12 hours. Take uninfected cells as MOCK. PKR, phosphorylated PKR, eIF2α, and phosphorylated eIF2α expression were detected by western blotting. β‐Actin was used as a protein loading control. (B) HeLa cells were treated with poly (I:C) (10 μg/mL) or infected with SPPV Gulang/2009 at an MOI of 3 for 12 hours. IFN‐β mRNA levels in the cell lysates were quantified by real‐time PCR. (C) HeLa cells were transfected with the siRNA specific for PKR (75 pmol/mL) or control siRNA for 48 h and then infected with SPPV Gulang/2009 at an MOI of 3. RNA interference effects for PKR in cells were detected by western blot. (D) HeLa cells transfected with siRNAs were infected with SPPV Gulang/2009 at an MOI of 3. IFN‐β mRNA levels in the cell lysates were quantified by real‐time PCR at 12 hpi. Data are represented as mean ± SEM; n = 3. Representative of three independent experiments. Significance was analyzed using two‐tailed Student's t‐test. **P < 0.01; ***P < 0.001. ns, not significant.