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. 2018 Sep 26;27(2):276–291. doi: 10.1111/1744-7917.12637

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© 2018 The Authors. Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Phylogenetic relationship of UGT201D3 with uridine diphosphate‐glycosyltransferase (UGT) family of Tetranychus urticae. The phylogram was generated using the maximum likelihood method in MEGA 5.05 and the inferred phylogeny was tested by bootstrap analysis with 1000 replications. The sequences used for constructing the tree are listed in Table S2. Filled circles indicate the newly cloned UGT gene of T. cinnabarinus. Filled triangles mean the other two UGT genes of T. cinnabarinus were used to analyze whether there are off‐target effects in RNA interference (RNAi).