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. 2018 Sep 26;27(2):276–291. doi: 10.1111/1744-7917.12637

Figure 6.

Figure 6

SDS‐PAGE (A) and western blotting (B) analysis of recombinant UGT201D3 expression in Escherichia coli (DE3). Lane M: protein markers; Lane 1: PET‐28a with isopropyl b‐D‐1‐thiogalactopyranoside (IPTG) induction; Lane 2: PET‐28a‐UGT201D3q with IPTG induction; Lane 3: soluble protein; Lane 4: purified by Ni2+‐NTA.