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Further investigation of effect of THD on MAPKs signaling pathway. Gene expression of IL-6, IL-10, and TNF-α was detected by RT-PCR (A–C). IL-6, IL-10, and TNF-α production in the supernatant was examined through ELISA kits (D–F). SB means p38 inhibitor, SP means JNK inhibitor, PD means ERK1/2 inhibitor. Data are expressed as mean ± SD. **P < 0.01 versus group without LPS and inhibitors; ##P < 0.01 versus group with LPS only.
