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. 2020 Jul 17;11:1065. doi: 10.3389/fphar.2020.01065

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Copyright © 2020 Xing, Fu, Cheng, Cai, Wang, Deng, Gong and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hyperoside regulation of the PHLPP2-AKT-GSK-3β signaling pathway in liver L02 cells. (A) Western blots. The cytoplasmic HO-1, p-GSK-3β, p-AKT, and PHLPP2 were analyzed using western blotting in liver L02 cells after treated with t-BHP. (B) Representative blots of cytoplasmic and nuclear Nrf2. (C) Quantification data of HO-1, cytoplasmic Nrf2, nuclear Nrf2, p-Nrf2 (S40), p-GSK-3β, p-AKT (S473), and PHLPP2. These western blots were quantified by using Image J Software and the data are expressed as mean and standard deviation (SD) and statistically analyzed by ANOVA. ^aP < 0.05 vs. Control, ^bP < 0.05 vs. Morin, ^cP < 0.05 vs. Hyp for 1 h, and ^dP < 0.05 vs. Hyp for 3 h.