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. 2020 Jul 17;11:1581. doi: 10.3389/fmicb.2020.01581

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Copyright © 2020 Li, Zhang, Pei, Xue, Ruan and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Virus detection results from the MRT-qPCR assay of the 1,187 clinical samples. For the spleen (A,B), bursa of Fabricius (C,D), and thymus (E,F) samples, the Ct value of positive samples for each virus are shown with scatter plots (A,C,E). Each dot represents the mean Ct value of three duplicate in a positive sample. Coinfection samples are shown with Venn diagrams (B,D,F). (G) Distribution of Ct values of CIAV in three tissues under simple infection and coinfection. Each dot represents the mean Ct value of three duplicates in a positive sample. The Ct values of CIAV single infection are shown with black dots. Coinfection of CIAV with different viruses is shown with gray dots.