TABLE 1.
Virus |
The SRT-qPCR |
The MRT-qPCR |
||||||
Slopea | Y-interceptb | R2c | Ed | Slope | Y-intercept | R2 | E | |
MDV | –3.04 | 37.79 | 0.9946 | 113.27 | –3.07 | 38.52 | 0.992 | 111.71 |
REV | –3.25 | 39.24 | 0.9949 | 103.09 | –3.09 | 39.40 | 0.9994 | 110.68 |
ARV | –3.36 | 40.32 | 0.9935 | 98.44 | –3.19 | 40.03 | 0.9986 | 105.82 |
CIAV | –3.15 | 39.62 | 0.9944 | 107.71 | –3.08 | 39.05 | 0.9974 | 111.19 |
FAdV | –3.24 | 41.06 | 0.9956 | 103.54 | –3.10 | 41.01 | 0.995 | 110.17 |
IBDV | –3.17 | 39.98 | 0.9926 | 106.76 | –3.27 | 41.31 | 0.995 | 102.21 |
aSlopes of standard curves. bThe intersection of the standard curve and the y-axis. cCorrelation coefficients (R2) of standard curves. dAmplification efficiencies (E) of the SRT-qPCR and MRT-qPCR assays.