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. 2020 Jul 17;11:1581. doi: 10.3389/fmicb.2020.01581

TABLE 1.

The sensitivities of the SRT-qPCR and MRT-qPCR assays, based on standard curves.

Virus The SRT-qPCR
The MRT-qPCR
Slopea Y-interceptb R2c Ed Slope Y-intercept R2 E
MDV –3.04 37.79 0.9946 113.27 –3.07 38.52 0.992 111.71
REV –3.25 39.24 0.9949 103.09 –3.09 39.40 0.9994 110.68
ARV –3.36 40.32 0.9935 98.44 –3.19 40.03 0.9986 105.82
CIAV –3.15 39.62 0.9944 107.71 –3.08 39.05 0.9974 111.19
FAdV –3.24 41.06 0.9956 103.54 –3.10 41.01 0.995 110.17
IBDV –3.17 39.98 0.9926 106.76 –3.27 41.31 0.995 102.21

aSlopes of standard curves. bThe intersection of the standard curve and the y-axis. cCorrelation coefficients (R2) of standard curves. dAmplification efficiencies (E) of the SRT-qPCR and MRT-qPCR assays.