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. 2020 Jul 17;10:1034. doi: 10.3389/fonc.2020.01034

Figure 1.

Figure 1

LncRNA DANCR is upregulated in colon cancer and contributes to cisplatin resistance. (A) Quantitative RT-PCR results of the DANCR expressions in 35 normal colon tissues and 35 tumor tissues. (B) Quantitative RT-PCR results of the DANCR expressions in normal colon epithelial cell and five colon cancer cells. (C) DANCR overexpression vector or control vector was transfected into HT-29 and SW620 cells for 48 h. The expressions of DANCR were detected by qRT-PCR. (D) HT-29 and (E) SW620 cells with control or DANCR overexpression vector transfection were treated with cisplatin at the indicated concentrations for 48 h. Cell viability was measured by MTT assay. (F) Establishment of cisplatin-resistant cell line from SW480 cells. The resistance was assessed by treatments with cisplatin at the indicated concentrations. (G) The expressions of DANCR were detected from SW480 parental and cisplatin-resistant cells by qRT-PCR. (H) SW480/CDDP R cells were transfected with control shRNA or DANCR shRNA for 48 h, the expressions of DANCR were detected by qRT-PCR. (I) The above cells were treated with cisplatin at 0, 5, 10, 20, 30, or 40 μM for 48 h; cell viabilities were measured by MTT assay. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001.