Figure 4.

DANCR and miR-125b-5p oppositely regulate glycolysis of gastric cancer cells. (A) Glucose uptake and (B) lactate product were measured in SW480 parental and cisplatin-resistant cells. (C) Protein expressions of HK2, LDHA were detected in SW480 parental, and cisplatin-resistant cells by Western blot. β-Actin was an internal control. (D) SW480 cisplatin-resistant cells were cotreated with control or oxamate at 10 mM plus cisplatin at the indicated concentrations for 48 h; the cell viability was measured by MTT assay. (E) HT-29 and (F) SW620 cells were transfected with control vector or DANCR overexpression vector for 48 h; the glucose uptake and lactate product were measured. (G) HT-29 and (H) SW620 cells were transfected with control miR or miR-125b-5p precursor for 48 h; the glucose uptake and lactate product were measured. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001.