Figure 6.

Restoration of HK2 in miR-125b-5p–overexpressing cells recovers glycolysis and cisplatin resistance. (A) HT-29 cells were transfected with control miR, miR-125b-5p alone, or miR-125b-5p plus HK2; the expressions of HK2 were measured by Western blot. β-Actin was an internal control. (B) The glucose uptake and lactate product assays were performed from the above cells. (C) The mRNA expressions of GLUT1 and LDHA were measured from the above cells. (D) HT-29, (E) SW620, and (F) SW480/CDDP R cells were transfected with control miR, miR-125b-5p alone, or miR-125b-5p plus HK2; cells were treated with cisplatin at the indicated concentrations. Cell viability was measured by MTT assay. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001.