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. 2020 Jul 17;8:559. doi: 10.3389/fcell.2020.00559

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Copyright © 2020 Wang, Zheng, Pan, Yao, Gao, Wang and Ding.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of icariin on the generation of ECM in STZ-induced diabetic rat. (A) The histopathology analysis of glomeruli using HE staining (200×). (B) The histopathology analysis of glomeruli using PAS staining (200×). (C) The histopathology analysis of glomeruli using Masson staining (200×). (D) Expression of FN in glomeruli through immunohistochemical (200×). (E) Glomerular surface area and glomerular tuft area were quantified using 30 glomeruli per mouse using ImageJ Software. (F) Expression of FN protein expression by western blot. Data are expressed as mean ± SD. *p < 0.05, **p < 0.01, compared with the normal control group; ^#p < 0.05, ^##p < 0.01, compared with the Model group.