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. 2020 Jul 17;8:559. doi: 10.3389/fcell.2020.00559

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Copyright © 2020 Wang, Zheng, Pan, Yao, Gao, Wang and Ding.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of icariin on Nrf2 activation and ECM generation in DN rats and HMC induced by high glucose. (A) Effect of icariin on the total expressions of Nrf2 in DN rats was detected by western blot assy. (B) Effect of icariin on Nrf2 protein level in DN rats was detected by immunohistochemical staining. Scale bar: 50 μm (C) Effect of icariin on Nrf2 protein level in high glucose induced HMC was detected by western blot assay. (D) Effect of icariin on the nuclear expressions of Nrf2 in STZ-induced diabetic rat kidney and high glucose induced HMC was detected by western blot assay. (E) Distribution of Nrf2 in HMC through Immunofluorescence. Bar: 20 μm. green fluorescence indicates localization of Nrf2. (F) Transcriptional activity of Nrf2 was measured by luciferase reporter assay. (G) DNA binding activity of Nrf2 was determined by electrophoretic mobility shift assay. (H) Effect of siNrf2 and ML385 on the inhibition of FN by icariin in HMC. Data are expressed as mean ± SD. **p < 0.01, compared with the normal control group; ^#p < 0.05, ^##p < 0.01, compared with the Model group or HG group; ^$$p < 0.01, compared with the icariin group.