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Axin2 mRNA in Saos-2 cells. Saos-2 cells were treated with (A) baicalein (35 μM), (B) miR-25 inhibitor (with or without baicalein) and (C) miR-25 mimic for 48 h. Then expressions were detected by qRT-PCR. GAPDH was used as an endogenous control. Values represent the mean ± standard deviation of 3 independent experiments performed in triplicate. *P < 0.05; **P < 0.01; ***P < 0.001 indicated statistical significance.
