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. Author manuscript; available in PMC: 2021 Jun 16.
Published in final edited form as: Biochemistry. 2020 Jun 4;59(23):2171–2181. doi: 10.1021/acs.biochem.0c00176

Figure 3.

Figure 3.

Competition binding assays demonstrated that fOSMR-ECD, fIL31RA-1FNIII, and mAb#1 share an overlapping epitope using SPR and YSD. (a.) SPR sensorgrams showing a decrease in the binding signal when fOMSR-ECD and mAb#1 were captured on the surface. The control was in the presence of 1x HBS-EP buffer. (b.) Percentage of inhibition for yeast cells labeled with either non-biotinylated mAb#1 or non-biotinylated fOSMR-ECD at twelve times their respective Kd values and subsequently labeled with mAb#1, fOSMR-ECD, and fIL31RA-1FNIII at Kd values (error bars, standard error of the mean, n≥3). Inhibition of mAb#1 by non-biotinylated OSMR was not determined.