Figure 7.

HCVc promotes the development of G‐MDSCs through ERK/IL‐6/STAT3 signalling. A, STAT3 phosphorylation in PBMCs treated with HCVc and/or rhIL‐10 for 30 min was evaluated by western blotting. Inhibition of STAT3 phosphorylation in MDSCs treated with S31‐201 was evaluated by western blotting. B, The results of three independent experiments showing a similar trend. C, Addition of rhIL‐10 improved the HCVc‐induced increase in Arg‐1, COX‐2 and iNOS. S31‐201 could inhibit the STAT3 phosphorylation stimulated by HCVc and rhIL‐10 and significantly decrease the levels of Arg‐1, COX‐2 and iNOS in the supernatant. D, IL‐10 elevated the expression of p‐STAT3, and Arg‐1 in G‐MDSCs of CHC patients, and their progression could be dramatically inhibited by S31‐201