Table 1.
Proteins associated with the T. ni brush border or epithelial cell membrane
| Contig name | Protein description† | Signal peptide‡ | Transmembrane domains | GPI signal |
|---|---|---|---|---|
| 1. Enzymes | ||||
| 1.1 Protein metabolism | ||||
| T_ni_cDNA_15821 | Membrane alanyl aminopeptidase | Y | 0 | Y |
| T_ni_cDNA_15865 | Membrane alanyl aminopeptidase | ND | 1 | Y |
| T_ni_cDNA_15923 | Membrane alanyl aminopeptidase | ND | 1 | N |
| T_ni_cDNA_15756 | Membrane alanyl aminopeptidase | ND | 0 | Y |
| T_ni_cDNA_15581 | Aminopeptidase N | Y | 1 | Y |
| T_ni_cDNA_15581 | Aminopeptidase N | Y | 1 | Y |
| T_ni_cDNA_15841 | Zinc carboxypeptidase | ND | 0 | Y |
| T_ni_cDNA_16326 | Zinc carboxypeptidase | ND | 1 | N |
| T_ni_cDNA_11989 | Serine carboxypeptidase | N | 1 | N |
| T_ni_cDNA_9354 | Dipeptidyl peptidase 4 | ND | 1 | N |
| T_ni_cDNA_2674 | Dipeptidyl peptidase 4 | N | 1 | N |
| T_ni_cDNA_11394 | Trypsin‐like protease | ND | 1 | N |
| T_ni_cDNA_16839 | Alkaline c‐like | ND | 1 | N |
| T_ni_cDNA_15309 | Alkaline c‐like | Y | 1 | N |
| T_ni_cDNA_14829 | Gamma‐glutamyltranspeptidase 1 | N | 1 | N |
| T_ni_cDNA_11454 | Angiotensin‐converting enzyme‐like | ND | 2 | N |
| 1.2 Lipid metabolism | ||||
| T_ni_cDNA_15964 | Bile salt‐activated lipase | ND | 1 | N |
| T_ni_cDNA_15786 | Pancreatic triacylglycerol lipase | ND | 1 | N |
| T_ni_cDNA_6516 | Group XV phospholipase A2 | Y | 1 | N |
| 1.3 Alkaline phosphatases | ||||
| T_ni_cDNA_16197 | Membrane‐bound alkaline phosphatase | Y | 0 | Y |
| T_ni_cDNA_7523 | Membrane‐bound alkaline phosphatase | ND | 0 | Y |
| T_ni_cDNA_10461 | Alkaline phosphatase | Y | 1 | N |
| 1.4 Esterases | ||||
| T_ni_cDNA_7581 | FE4 esterase | ND | 0 | Y |
| T_ni_cDNA_5769 | FE4 esterase | ND | 1 | N |
| 2. Transporters | ||||
| 2.1 Nutrient transporters | ||||
| T_ni_cDNA_4704 | Facilitated trehalose transporter TreT1 | N | 12 | N |
| T_ni_cDNA_16594 | Facilitated trehalose transporter TreT1 | ND | 5 | N |
| T_ni_cDNA_16422 | Facilitated trehalose transporter TreT1 | ND | 4 | N |
| T_ni_cDNA_16750 | Facilitated trehalose transporter TreT1 | N | 4 | N |
| T_ni_cDNA_6038 | Facilitated trehalose transporter TreT1 | ND | 7 | N |
| T_ni_cDNA_2642 | Facilitated trehalose transporter TreT1 | ND | 6 | N |
| T_ni_cDNA_8414 | Facilitated trehalose transporter TreT1 | ND | 4 | N |
| T_ni_cDNA_18685 | Facilitated trehalose transporter TreT1 | ND | 4 | N |
| T_ni_cDNA_5339 | Facilitated trehalose transporter TreT1 | N | 12 | N |
| T_ni_cDNA_10089 | Facilitated trehalose transporter TreT1 | Y | 12 | N |
| T_ni_cDNA_6537 | Facilitated trehalose transporter TreT1 | N | 12 | N |
| T_ni_cDNA_1150 | Facilitated trehalose transporter TreT1‐2 | ND | 12 | N |
| T_ni_cDNA_15922 | Facilitated trehalose transporter TreT1‐2 | N | 12 | N |
| T_ni_cDNA_15135 | Facilitated trehalose transporter TreT1‐2 | N | 12 | N |
| T_ni_cDNA_2589 | Facilitated trehalose transporter TreT1‐2 | N | 12 | N |
| T_ni_cDNA_16456 | Monocarboxylate transporter 9 | ND | 7 | N |
| T_ni_cDNA_15428 | Monocarboxylate transporter 12 isoform x1 | ND | 12 | N |
| T_ni_cDNA_7342 | Monocarboxylate transporter 12 | ND | 5 | N |
| T_ni_cDNA_7344 | Monocarboxylate transporter 12 | ND | 5 | N |
| T_ni_cDNA_936 | Monocarboxylate transporter 13 | N | 12 | N |
| T_ni_cDNA_15711 | Peptide transporter family 1 | ND | 10 | N |
| T_ni_cDNA_331 | Sodium‐dependent amino acid transporter 1 | ND | 10 | N |
| T_ni_cDNA_15560 | Sodium‐dependent amino acid transporter 1 | N | 11 | N |
| T_ni_cDNA_268 | Proton‐coupled amino acid transporter 4 | N | 10 | N |
| T_ni_cDNA_10643 | b(+)‐Type amino acid transporter 1 | ND | 12 | N |
| T_ni_cDNA_11849 | Neutral and basic amino acid transport protein RBAT | N | 1 | N |
| T_ni_cDNA_484 | p Protein (tyrosine transport – melanin biosynthesis) | N | 4 | N |
| T_ni_cDNA_16680 | Carnitine transporter (solute carrier family 22 member 5) | ND | 6 | N |
| T_ni_cDNA_15116 | Long‐chain fatty acid transport protein 4 | ND | 4 | N |
| T_ni_cDNA_2856 | Long‐chain fatty acid transport protein 4 | N | 2 | N |
| T_ni_cDNA_16312 | Folate transporter (solute carrier family 46 member 3) | ND | 7 | N |
| T_ni_cDNA_13650 | Folate transporter (solute carrier family 46 member 3) | N | 4 | N |
| T_ni_cDNA_2107 | Folate transporter (solute carrier family 46 member 3) | N | 11 | N |
| T_ni_cDNA_13629 | Folate transporter (solute carrier family 46 member 3) | ND | 5 | N |
| T_ni_cDNA_14535 | Thiamine transporter 1 | N | 9 | N |
| T_ni_cDNA_6134 | Sodium‐dependent multivitamin transporter | N | 13 | N |
| T_ni_cDNA_14772 | Glucose transporter type 1 | N | 10 | N |
| T_ni_cDNA_16283 | sodium‐ and chloride‐dependent glycine transporter 1 | ND | 10 | N |
| T_ni_cDNA_4340 | Equilibrative nucleoside transporter 1 | N | 10 | N |
| T_ni_cDNA_11410 | Nucleoside transporter (solute carrier family 28 member 3) | N | 6 | N |
| 2.2 Ion transporters | ||||
| T_ni_cDNA_7971 | Monoamine transporter (solute carrier family 22 member 3) | N | 10 | N |
| T_ni_cDNA_10297 | Monoamine transporter (solute carrier family 22 member 3) | N | 9 | N |
| T_ni_cDNA_16742 | Organic cation transporter (unknown specificity) | N | 7 | N |
| T_ni_cDNA_16932 | Organic cation transporter (unknown specificity) | ND | 3 | N |
| T_ni_cDNA_15514 | Organic cation transporter (unknown specificity) | ND | 8 | N |
| T_ni_cDNA_16584 | Organic cation transporter (unknown specificity) | ND | 1 | N |
| T_ni_cDNA_16504 | Organic anion transporter (solute carrier family 22 member 6) | N | 12 | N |
| T_ni_cDNA_4393 | Zinc transporter zip1 | N | 8 | N |
| T_ni_cDNA_15008 | Zinc transporter zip1 | N | 7 | N |
| T_ni_cDNA_15544 | Zinc transporter 1 | N | 5 | N |
| T_ni_cDNA_15075 | Zinc transporter 2 | ND | 2 | N |
| T_ni_cDNA_5548 | Zinc transporter 5 | N | 6 | N |
| T_ni_cDNA_4830 | Zinc transporter 8 | N | 6 | N |
| T_ni_cDNA_8634 | Sodium potassium‐transporting ATPase subunit alpha | N | 8 | N |
| T_ni_cDNA_5157 | Sodium potassium‐transporting ATPase subunit beta‐1 | N | 1 | N |
| T_ni_cDNA_15599 | Sodium potassium calcium exchanger 4 | Y | 11 | N |
| T_ni_cDNA_16457 | Otopetrin‐2‐like (calcium) | ND | 2 | N |
| T_ni_cDNA_8680 | Plasma membrane calcium‐transporting ATPase 2 | N | 10 | N |
| T_ni_cDNA_5242 | Membrane magnesium transporter 1 | N | 2 | N |
| T_ni_cDNA_4851 | Metal transporter CNNM2 | ND | 4 | N |
| T_ni_cDNA_2047 | Metal transporter CNNM4 | Y | 5 | N |
| T_ni_cDNA_16108 | High‐affinity copper uptake protein 1 | N | 1 | N |
| T_ni_cDNA_2666 | High‐affinity copper uptake protein 1 | N | 2 | N |
| T_ni_cDNA_1242 | Copper‐transporting ATPase 1 | ND | 8 | N |
| T_ni_cDNA_5956 | Sodium‐independent sulfate anion transporter | ND | 2 | N |
| T_ni_cDNA_2292 | Sodium‐independent sulfate anion transporter | N | 9 | N |
| T_ni_cDNA_17245 | Inorganic phosphate cotransporter | N | 1 | N |
| T_ni_cDNA_9286 | Inorganic phosphate cotransporter | ND | 2 | N |
| T_ni_cDNA_16781 | Inorganic phosphate cotransporter | ND | 5 | N |
| T_ni_cDNA_16392 | Inorganic phosphate cotransporter | ND | 5 | N |
| T_ni_cDNA_11064 | Inorganic phosphate cotransporter | ND | 4 | N |
| T_ni_cDNA_11851 | Inorganic phosphate cotransporter | N | 10 | N |
| T_ni_cDNA_14148 | Inorganic phosphate cotransporter | N | 10 | N |
| T_ni_cDNA_8012 | Band 3 anion transport protein | ND | 3 | N |
| T_ni_cDNA_8012 | Band 3 anion transport protein | ND | 7 | N |
| T_ni_cDNA_11471 | Band 3 anion transport protein | ND | 1 | N |
| T_ni_cDNA_8298 | Bestrophin‐4 (chloride transport) | ND | 5 | N |
| T_ni_cDNA_49 | Transmembrane channel‐like protein 5 | N | 9 | N |
| T_ni_cDNA_6160 | Transmembrane channel‐like protein 7 | ND | 2 | N |
| T_ni_cDNA_5307 | Transmembrane channel‐like protein 7 | ND | 1 | N |
| T_ni_cDNA_3081 | v‐Type proton ATPase subunit s1 | Y | 2 | N |
| T_ni_cDNA_2854 | v‐Type proton ATPase 16 kDa proteolipid subunit | N | 4 | N |
| T_ni_cDNA_16138 | v‐Type proton ATPase 116 kDa subunit a | N | 6 | N |
| T_ni_cDNA_8655 | v‐Type proton ATPase 116 kDa subunit a | N | 7 | N |
| 2.3 ATP‐binding cassette (ABC) and major facilitator (MFS) superfamily transporters | ||||
| T_ni_cDNA_16946 | ABC sub‐family A member 3 | ND | 1 | N |
| T_ni_cDNA_6541 | ABC sub‐family G member 4 | ND | 1 | N |
| T_ni_cDNA_7803 | ABC sub‐family G member 4 | N | 6 | N |
| T_ni_cDNA_12415 | ABC sub‐family G member 4 | N | 7 | N |
| T_ni_cDNA_6541 | ABC sub‐family G member 4 | ND | 5 | N |
| T_ni_cDNA_9811 | Multidrug resistance protein 1a | ND | 2 | N |
| T_ni_cDNA_7617 | Multidrug resistance‐associated protein lethal 03659 | N | 11 | N |
| T_ni_cDNA_16958 | Multidrug resistance‐associated protein lethal 03659 | ND | 2 | N |
| T_ni_cDNA_7620 | Multidrug resistance‐associated protein 4 | N | 6 | N |
| T_ni_cDNA_1434 | Multidrug resistance‐associated protein lethal 03659 | N | 6 | N |
| T_ni_cDNA_9811 | Multidrug resistance protein homolog 49 isoform x1 | ND | 4 | N |
| T_ni_cDNA_17333 | Multidrug resistance protein homolog 49 | ND | 6 | N |
| T_ni_cDNA_14948 | Multidrug resistance‐associated protein 7 | ND | 9 | N |
| T_ni_cDNA_3976 | Multidrug resistance‐associated protein 4 | ND | 4 | N |
| T_ni_cDNA_7619 | Multidrug resistance‐associated protein 4 | ND | 5 | N |
| T_ni_cDNA_3976 | Multidrug resistance‐associated protein 4 | ND | 5 | N |
| T_ni_cDNA_16436 | Multidrug resistance‐associated protein lethal 03659 | ND | 6 | N |
| T_ni_cDNA_11576 | Multidrug resistance‐associated protein lethal 03659 | N | 2 | N |
| T_ni_cDNA_3465 | Major facilitator superfamily domain‐containing protein 6 | N | 10 | N |
| 2.4 Other transporters | ||||
| T_ni_cDNA_16176 | Nose resistant to fluoxetine protein 6 | ND | 5 | N |
| T_ni_cDNA_16598 | Nose resistant to fluoxetine protein 6 | ND | 3 | N |
| T_ni_cDNA_16620 | Nose resistant to fluoxetine protein 6 | ND | 4 | N |
| T_ni_cDNA_16176 | Nose resistant to fluoxetine protein 6 | ND | 4 | N |
| T_ni_cDNA_16467 | Nose resistant to fluoxetine protein 6 | ND | 6 | N |
| T_ni_cDNA_11792 | sid1 Transmembrane family member 1 | Y | 7 | N |
| T_ni_cDNA_1279 | sid1 Transmembrane family member 1 | ND | 4 | N |
| T_ni_cDNA_13757 | sid‐1‐Related gene3 precursor | ND | 3 | N |
| T_ni_cDNA_2198 | Aquaporin | N | 6 | N |
| T_ni_cDNA_16275 | Aquaporin | N | 5 | N |
| T_ni_cDNA_6828 | Cell cycle control protein 50a (phospholipid transport—flippase) | ND | 1 | N |
| T_ni_cDNA_6917 | Ileal sodium bile acid cotransporter‐like | Y | 9 | N |
| 3. Signaling and development | ||||
| T_ni_cDNA_4126 | G‐protein coupled receptor 125 | ND | 5 | N |
| T_ni_cDNA_5794 | G‐protein coupled receptor mth2‐like | ND | 7 | N |
| T_ni_cDNA_10188 | Notch protein isoform X1 | ND | 1 | N |
| T_ni_cDNA_8224 | Dispatched protein (hedgehog signaling) | N | 12 | N |
| T_ni_cDNA_5965 | Patched domain‐containing protein 3‐like (hedgehog receptor) | ND | 9 | N |
| T_ni_cDNA_15629 | Fasciclin‐2 isoform x1 (adhesion protein—inhibitor of EGFR signaling) | ND | 0 | Y |
| T_ni_cDNA_15628 | Fasciclin‐2 isoform x3 | Y | 2 | N |
| T_ni_cDNA_10096 | Fasciclin‐3 isoform x3 | Y | 1 | N |
| T_ni_cDNA_8856 | Plexin‐a4 | ND | 2 | N |
| T_ni_cDNA_6561 | Plexin domain‐containing protein 2‐like | N | 1 | N |
| T_ni_cDNA_16287 | Protein rolling stone‐like | N | 6 | N |
| 4. Cell–cell interaction | ||||
| T_ni_cDNA_1567 | Integrin beta‐nu (midgut cell development) | ND | 1 | N |
| T_ni_cDNA_1572 | Integrin beta‐nu | ND | 1 | N |
| T_ni_cDNA_6146 | Integrin alpha‐ps1 isoform x1 | ND | 1 | N |
| T_ni_cDNA_889 | Integrin alpha‐ps2‐like | Y | 1 | N |
| T_ni_cDNA_1079 | Dystroglycan isoform x1 | ND | 1 | N |
| T_ni_cDNA_16036 | Cadherin‐like membrane protein precursor | ND | 1 | N |
| T_ni_cDNA_15320 | De‐cadherin‐like isoform x2 | ND | 1 | N |
| T_ni_cDNA_13185 | Lachesin‐like isoform x1 | ND | 1 | N |
| T_ni_cDNA_1960 | Innexin inx2 (gap junctions) | N | 4 | N |
| 3.3 Other | 1 | N | ||
| T_ni_cDNA_5683 | 23 kDa integral membrane protein | N | 4 | N |
| T_ni_cDNA_15216 | 23 kDa integral membrane protein | N | 4 | N |
| T_ni_cDNA_5730 | Protein mesh isoform x2 | ND | 1 | N |
| T_ni_cDNA_6939 | Basigin | ND | 2 | N |
| 5. Other proteins | ||||
| 5.1 Immunity/defense | ||||
| T_ni_cDNA_3905 | Peptidoglycan‐recognition protein sc2‐like | N | 1 | N |
| T_ni_cDNA_3908 | Peptidoglycan‐recognition protein 2‐like | N | 1 | N |
| T_ni_cDNA_14656 | Croquemort‐like isoform x3 | N | 2 | N |
| T_ni_cDNA_11150 | Transmembrane protein 120 | ND | 1 | N |
| T_ni_cDNA_6486 | Transmembrane protein 120 | ND | 5 | N |
| 5.2 Chitin metabolism | ||||
| T_ni_cDNA_3621 | Trehalase‐like isoform x1 | Y | 1 | N |
| T_ni_cDNA_8547 | Chitinase‐3‐like protein 2 | N | 1 | N |
| T_ni_cDNA_13856 | Laccase‐1‐like isoform x2 | ND | 0 | Y |
| 5.3 Other | ||||
| T_ni_cDNA_9349 | Protein yellow | Y | 0 | Y |
| T_ni_cDNA_2522 | Protein yellow‐like | ND | 0 | Y |
| T_ni_cDNA_1605 | Transmembrane protein 87a | Y | 7 | N |
| T_ni_cDNA_8393 | Transmembrane protein 177 | ND | 2 | N |
| T_ni_cDNA_5627 | Transmembrane protein 222 | N | 2 | N |
| T_ni_cDNA_7343 | Transmembrane protein 256 homolog isoform x1 | ND | 3 | N |
| T_ni_cDNA_11297 | Leucine‐rich transmembrane protein (aael003720) | ND | 1 | N |
| T_ni_cDNA_9865 | Neutral ceramidase isoform x1 | N | 1 | N |
| T_ni_cDNA_12371 | Leucine‐rich repeat neuronal protein 3‐like isoform x1 | Y | 1 | N |
| T_ni_cDNA_8987 | Platelet glycoprotein v | Y | 1 | N |
| T_ni_cDNA_15370 | Motile sperm domain‐containing protein 2‐like | N | 2 | N |
| T_ni_cDNA_8049 | Cleft lip and palate transmembrane protein 1 homolog | N | 4 | N |
| T_ni_cDNA_13005 | Cklf‐like marvel transmembrane domain‐containing protein 4 | N | 4 | N |
| T_ni_cDNA_3867 | Sel1 (signal transduction) or Skt5p (chitin synthesis) | ND | 1 | N |
| T_ni_cDNA_5900 | TM2‐domain (related to 7 transmembrane GPCR) | ND | 2 | N |
| T_ni_cDNA_14902 | Vacuolar ATPase assembly integral membrane VMA21 | ND | 2 | N |
| T_ni_cDNA_6626 | Uncharacterized insect protein (two protein‐binding domains) | ND | 1 | N |
| T_ni_cDNA_7146 | Uncharacterized insect protein (two protein‐binding domains) | N | 2 | N |
| T_ni_cDNA_11766 | Uncharacterized insect protein (NLS) | ND | 1 | N |
| T_ni_cDNA_16269 | Uncharacterized insect protein | ND | 0 | Y |
†Annotation based on Gene Ontology and BLAST analysis.
‡ND: could not be determined as 5′ open‐reading frame of the contig did not extend to the start codon; N: No; Y: Yes.