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. 2018 Dec 12;111(2):495–513. doi: 10.1111/mmi.14170

Figure 4.

Figure 4

XisF4 induces the expression of PA0727. A. Start sites of four putative promoter regions of the related operons in Pf4 are indicated with arrows, with P1 for xisF4‐PA0719, P2 for PA0720‐0723, P3 for PA0724‐0726 and P4 for PA0727‐0728. B. Relative mRNA levels of the first gene (except PA0717) of the putative operons shown in A in PAO1/pHERD20T‐xisF4 versus PAO1/pHERD20T. C. The lacZ reporter activities were determined in strain PAO1 carrying pHERD20T and pHERD20T‐xisF4. When cells were grown to OD600 ~ 0.1, 10 mM arabinose was added for 3 h of induction. Three independent cultures were used, and error bars indicate standard deviation in B and C. D. EMSA showed that XisF4 bound to the promoter regions of P1 and P4 in a concentration‐dependent manner, but XisF4 did not bind to the promoter regions of P2 or P3.