Table 2.
Integrated diagnosis of supratentorial ependymomas.
| Case | Age (y) | Location | Histology | p65RelA IHC | RELA FISH | C11ORF95 FISH | Methylation assay | RT‐PCR | RNAseq | Integrated diagnosis | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Type | Grade | ||||||||||
| 1 | 17 | NA | Classic | III | 1 | 1 | 1 | Failed | ND | ND | RELA‐fused epend. |
| 2 | 3 | Temporo‐frontal | Clear‐cell | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 3 | 1 | Parietal | Clear‐cell | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 4 | 12 | NA | Clear‐cell | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 5 | 5 | Frontal | Clear‐cell | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 6 | 8 | Frontal | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 7 | 10 | Parietal | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 8 | 7 | Frontal | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 9 | 12 | Parietal | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 10 | 5 | Parietal | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 11 | 2 | Occipito‐parietal | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 12 | 5 | Fronto‐parietal | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 13 | 1 | NA | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 14 | 4 | NA | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 15 | 2 | Pineal | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 16 | 11 | Frontal | Classic | III | 1 | 1 | 1 | EPN, RELA | ND | ND | RELA‐fused epend. |
| 17 | 7 | Parietal | Classic | III | 1 | Doubtful | Doubtful | EPN, RELA | ND | ND | RELA‐fused epend. |
| 18 | 9 | Frontal | Classic | III | 1 | 1 | Failed | EPN, RELA | ND | ND | RELA‐fused epend. |
| 19 | 2 | Temporal | Classic | III | 1 | ND | ND | EPN, RELA | ND | ND | RELA‐fused epend. |
| 20 | 3 | Temporal | Classic | III | 1 | Failed | ND | EPN, RELA | ND | ND | RELA‐fused epend. |
| 21 | 2 | NA | Papillary | III | 1 | 1 | 1 | Undetermined | 0 | ND | RELA‐fused epend. |
| 22 | 9 | Undetermined | Classic | III | 1 | 1 | 0 | Undetermined | 0 | No fusion | RELA‐fused epend. |
| 23 | 1 | Thalamic‐intrav | Classic | III | 0 | 0 | 1 | EPN, RELA | 0 | ND | Non RELA/YAP epend. |
| 25 | 8 | Frontal | Classic | III | 0 | ND | ND | Undetermined | 0 | ND | Non RELA/YAP epend. |
| 24 | 1 | Tectal | Classic | III | 0 | Failed | ND | Undetermined | 0 | MAML2‐ASCL2 | Non RELA/YAP epend. |
| 34 | 10 | V3 | Classic | III | 0 | 0 | 0 | Undetermined | 0 | MARK2‐ADCY3 | Non RELA/YAP epend. |
| 35 | 11 | Temporo‐parietal | Papillary | III | 0 | 0 | 0 | Undetermined | ND | ND | Non RELA/YAP epend. |
| 36 | 2 | Frontal | Classic | III | 0 | Failed | Failed | Failed | ND | ND | Non RELA/YAP epend. |
| 27 | 2 | Temporo‐parietal | Classic | II | 0 | Failed | Failed | EPN, YAP1 | ND | ND | YAP‐fused ependymoma |
| 26 | 0,1 | Temporo‐parietal | Classic | III | 0 | 0 | Doubtful | EPN, YAP1 | ND | ND | YAP‐fused ependymoma |
| 37 | 10 | Parieto‐occipital | Mixed | III | 0 | 0 | Doubtful | Undetermined | ND | ND | Epend/subep.mixed tu |
| 38 | 15 | Intraventricular | Mixed | III | 0 | 0 | 0 | Undetermined | ND | ND | Epend/subep.mixed tu |
| 39 | 4 | Parietal | Mixed | II | 0 | 0 | 0 | Undetermined | ND | ND | Epend/subep.mixed tu |
| 40 | 14 | Parieto‐occipital | Mixed | III | 0 | Doubtful | 0 | Undetermined | ND | ND | Epend/subep.mixed tu |
Abbreviations: epend. = ependymoma; intra = intraventricular, ND = not done; Mixed = ependymal/subependymal mixed tumor; tu = tumor * 450K Max‐Score not in confidence interval.