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. 2018 Nov 9;19(1):206–220. doi: 10.1111/1755-0998.12956

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© 2018 The Authors. Molecular Ecology Resources Published by John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Heatmap of fish species and genera detected in samples taken from Dutch (E. Sch: Eastern Scheldt & W.Sch: Western Scheldt), UK (Mersey & Kent) and Portuguese (Minho & Aveiro) estuaries. The heatmap only shows taxa that are identified at the genus or species level. Fish taxa detected in Crangon crangon pooled stomach samples (St) are shown after DNA amplification with two markers: 12S and COI. Sediment samples (Se) are only shown after DNA amplification with the 12S primers since COI amplification resulted in the detection of only 1 MOTU (D. labrax) in 1 sample (Minho 1). Colours represent differences in relative read abundances, and the numbers below the columns show the total number of fish reads per sample that could be assigned to the species or genus level [Colour figure can be viewed at wileyonlinelibrary.com]