Table 1.
Comparison of T m values measured in duplicate for DERAEc by nanoDSF and by TSA in the presence of fluorescent SYPRO orange dye.a
| Mutation site | 30X | 31X | 55X | 59X | 157X | 188X | |
|---|---|---|---|---|---|---|---|
| X = Ile | nanoDSF | 63.9 ± 0.04 | 63.3 ± 0.07 | 63.9 ± 0.02 | 62.8 ± 0.03 | 64.1 ± 0.13 | 63.8 ± 0.13 |
| TSA | 62.9 ± 0.32 | 62.6 ± 0.14 | 63.3 ± 0.29 | 62.0 ± 0.00 | 63.3 ± 0.25 | 63.1 ± 0.38 | |
| X = Arg | nanoDSF | 62.5 ± 0.10 | 63.0 ± 0.04 | 62.6 ± 0.04 | 62.2 ± 0.16 | 60.2 ± 0.04 | 54.5 ± 0.19 |
| TSA | 60.9 ± 0.14 | 61.6 ± 0.14 | 60.4 ± 0.29 | 60.3 ± 0.00 | 58.7 ± 0.25 | 55.2 ± 0.38 | |
| X = Trp | nanoDSF | 62.0 ± 0.07 | n.a.b | 62.5 ± 0.28 | 61.0 ± 0.05 | 61.6 ± 0.07 | 56.2 ± 0.10 |
| TSA | 60.5 ± 0.00 | n.a. | 60.3 ± 0.14 | 59.9 ± 0.14 | 58.7 ± 0.43 | 55.0 ± 0.00 |
ananoDSF measures in 0.05 °C steps and values are unlikely to be identical, while the TSA assay is measured in 0.5 °C steps with interpolation; for matching T m values, there is no standard deviation. bClone not available.