Figure 6.

Induced expression of C‐type lectin domain protein (CTLDP) genes in Thitarodes xiaojinensis fat body by Ringer's (RS), Ophiocordyceps sinensis (Os), Cordyceps militaris (Cm) and Enterobacter cloacae (Ec) challenges. (A) Venn diagram demonstrating unique or common induction of a gene. The genes shown log₂‐fold change > 1 or log₂‐fold change < 0.5 after challenges were considered as differentially expressed genes and submitted to the Venny server (http://bioinfogp.cnb.csic.es/tools/venny/) for statistics. (B) Transcriptional changes of 32 candidate genes after different challenges. Gene boxes were colored based on the value of the log₂‐fold change. Cm/Os12, Cm/Os48 and Cm/Os72 represent the larvae infected with C. militaris/O. sinensis for 12, 48 and 72 h. Os1yr represents the larvae infected with O. sinensis for a year. (C) Confirmation of differentially expressed genes by quantitative real‐time polymerase chain reaction. T. xiaojinensis ribosomal protein S3 (rpS3) was used as an internal control to normalize the templates. Messenger RNA levels are normalized to the control group and expressed as log₂. Each untreated and treated group contained three larvae. The bars represent the means ± SEM (n = 3) from three replications and Student's t‐test was used to calculate the statistically significant difference among groups. ^* P < 0.05; ^** P < 0.01; ^*** P < 0.001.