Table 1.

Experimental design of functional analysis

	Pre-incubation 18 h					Incubation 12 h
	Control	Affected				Control	Affected
Blanks	DMSO 0.1%	0.01 µM ABZ	0.1 µM ABZ	1.0 µM ABZ	WASHING	DMSO 0.1%	DMSO 0.1%
1	DMSO 0.1%	0.01 µM ABZ	0.1 µM ABZ	1.0 µM ABZ		10 µM ABZ	10 µM ABZ
2	DMSO 0.1%	0.01 µM ABZ	0.1 µM ABZ	1.0 µM ABZ		10 µM ABZ	10 µM ABZ
3	DMSO 0.1%	0.01 µM ABZ	0.1 µM ABZ	1.0 µM ABZ		10 µM ABZ	10 µM ABZ

Affected groups of helminths were pre-incubated with sub-lethal concentrations of ABZ (0.01, 0.1 and 1.0 µM) for 18 h. Control groups were pre-incubated with no ABZ. Both affected and control groups were followingly incubated with high ABZ concentration (10 µM) for 12 h except the blanks.