Table 2.

Biotransformation of ABZ inH. contortusadults—the metabolites detected by UHPLC-MS/MS

tR (min)	Theoretical m/z values of [M+H]+ ions	Elemental composition	Description of metabolite formation		Product ions of [M+H]+, m/z	Metabolite designation
			Phase I	Phase II
2.0	444.14	C18H25N3O8S	S-oxidation	N-glycosidation	282, 240, 208	M1ABZ
2.5	444.14	C18H25N3O8S	S-oxidation	N-glycosidation	282, 240, 208	M2ABZ
3.4	282.09	C12H15N3O3S	S-oxidation	–	240, 208, 191,159	M3ABZ
4.3	370.14	C16H23N3O5S	Hydrolysis	N-glycosidation	208	M4ABZ
5.2	298.09	C12H15N3O4S	2*S-oxidation	–	266, 224, 159	M6ABZ
5.1	428.15	C18H25N3O7S	–	N-glycosidation	266, 234	M7ABZ
5.1	428.15	C18H25N3O7S	+O, hydrolysis	Glycosidation, O-acetylation	208	M11ABZ
5.3	428.15	C18H25N3O7S	+O, hydrolysis	Glycosidation, O-acetylation	208	M12ABZ
5.6	428.15	C18H25N3O7S	+O, hydrolysis	Glycosidation, O-acetylation	208	M13ABZ
6.1	428.15	C18H25N3O7S	+O, hydrolysis	Glycosidation, O-acetylation	208	M10ABZ
6.1	428.15	C18H25N3O7S	–	N-glycosidation	266, 234	M8ABZ
6.7	428.15	C18H25N3O7S	–	N-glycosidation	266, 234	M9ABZ
7.4	266.10	C12H15N3O2S	–	–	234	ABZ (parent drug)