Fig. 1.

Tdp-43 localizes in the nucleus and cytosol of motoneurons. a Spinal cord cross sections of adult CD-1 mice immunostained with an antibody against Tdp-43 and an antibody against choline acetyltransferase (ChAT) as a motoneuron marker. DAPI was used for visualization of nuclei. Scale bar: 10 μm. b Primary mouse motoneurons (DIV6) were immunostained with an antibody against Tdp-43 and with an antibody against α-Tubulin. DAPI was used for visualization of nuclei. Scale bar: 10 μm. c Immunostaining of motoneurons transduced with empty lentiviral expression vector (control) or vector expressing a shRNA targeting Tdp-43 (shTdp-43) with antibodies against Tdp-43 and Tubulin. Scale bar: 10 μm