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. 2020 Jul 17;14:182. doi: 10.3389/fncel.2020.00182

Figure 1.

Figure 1

Characterization of bone marrow mesenchymal stem cells (BMSCs) and exosomes from BMSCs (BMSC-Exos); BMSC-Exos increase cell viability following oxygen-glucose deprivation/reoxygenation (OGD/R). (A) Flow cytometry analyses indicated that BMSCs were positive for CD29, CD44, and CD90 but were negative for CD45. (B) Round morphologies of BMSC-Exos were demonstrated by tandem electron microscopy (TEM); scale bar = 100 nm. (C) Western blot analyses showed that the BMSC-Exos were positive for the specific exosome surface markers TSG101 and CD9. (D) NanoSight NTA analysis indicated that the diameters of BMSC-Exos were around 100 nm. (E) A significant reduction in cell viability was observed in OGD 6-h, 9-h, 12-h, and 24-h groups (n = 3). (F) BMSC-Exos enhanced cell viability in a dose-dependent manner (n = 3). (G) BMSC-Exos reduced lactate dehydrogenase (LDH) release following OGD/R (n = 3). *p < 0.05, ***p < 0.001 vs. control group; ##p < 0.01 vs. OGD/R group.