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. 2020 Jul 17;11:1476. doi: 10.3389/fimmu.2020.01476

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Copyright © 2020 Moore, Huang, Jain, Chalmers, Macian and Putterman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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T cell infiltration and subsets in MRL/lpr tissues. (A) Immunofluorescent staining for CD4 (green) and CD8 (red) T cell infiltration in choroid plexus (top) and salivary gland (bottom), magnification = 20X. (B) Percentage of CD4+ T cells (left), CD8+ T cells (center), and double negative T cells (right) in MRL/lpr tissues by flow cytometry; CP, choroid plexus; Gland, salivary gland; LN, lymph node. Values represent percentages of parent CD3+ T cells. (C) Sample clonality of MRL/lpr spleen (n = 9), salivary gland (n = 4), and choroid plexus tissues (n = 9). (D) Normalized number of unique productive clones in MRL/lpr tissue repertoires calculated by dividing the total number of unique productive clones to the amount of DNA analyzed. (E) Sum of the top 10 productive rearrangement frequencies. P-values were determined with one-way ANOVAs, *p < 0.05, **p < 0.01, ****p < 0.0001.

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