Figure 5.

Contribution of autophagy to the cytotoxicity of As^III combined with Tetra in MDA-MB-231 cells by modulating cell cycle progression. (A–D) After treatment with 10 μM As^III+6.4 μM Tetra in the presence or absence of 3-MA (0.25, 1.0 mM) or wortmannin (0.25, 1.0 μM) for 48 h, cell cycle profiling was performed by FACSCanto flow cytometer. Analyzed data and profiles for each G₀/G₁ and G₂/M phase using Diva software and ModFit LT™ ver.3.0. are shown in the gray area. Cells at S phase are shown as shaded area. A representative FACS histogram from three separate experiments is shown (A). *p<0.05, vs. control; ^§p<0.05, ^†p<0.01, vs. 10 μM As^III +Tetra 6.4 μM. As, As^III; Tetra, tetrandrine; Wort, wortmannin.