Figure 7.

Abrogation of the cytotoxicity of As^III combined with Tetra by JNK inhibition in MDA-MB-231 cells. Following the treatment for 48 h with 10 μM As^III and 6.4 μM Tetra, alone or in combination, in the presence or absence of MAPK inhibitors and their negative controls [JNK inhibitor SP600125 and its negative control SP600125NC (A); ERK inhibitor PD98059 (B); p38 MAPK inhibitor SB203580 and its negative control SB202474 (C)], the cell viability of MDA-MB-231 was determined by XTT assay. Relative cell viability was calculated as the ratio of the absorbance at 450 nm of each treatment group against those of the corresponding untreated control group. Data are shown as the means and SD from more than three independent experiments. *p<0.01 vs. the combination; ^§p<0.01, vs. 10 μM As^III alone; ^#p<0.01, vs. 6.4 μM Tetra alone. As, As^III; Tetra, tetrandrine; SP600, SP600125; SP600 NC, SP600125NC; SB203, SB203580; SB202, SB202474.