Figure 9.

Effect of SP600125 on the induction of autophagy in MDA-MB-231 cells treated with As^III combined with Tetra. After treatment with 10 μM As^III+6.4 μM Tetra in the presence or absence of 10 μM of SP600125 and its negative control SP600125NC for 48 h, the expression of LC3 protein was analyzed using western blot. (A) Representative images of the expression profile of LC3 are shown from three independent experiments. (B) The relative expression level was expressed as the ratio between LC3 protein and β-actin protein expression levels, and compared with those of untreated control group. Results are shown as the means ± SD from three independent experiments. *p<0.05 vs. control. As, As^III; Tetra, tetrandrine; SP600, SP600125; SP600 NC, SP600125NC.