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. 2018 May 17;45(2):119–140. doi: 10.1111/nan.12489

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© 2018 The Authors Neuropathology and Applied Neurobiology published by John Wiley & Sons Ltd on behalf of British Neuropathological Society

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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GAMM subpopulation analysis in GL‐261 implanted nonmyeloablative conditioned mice. (A) Subpopulation purity analysis using Ly6C/MHCII/MerTK/CD64 gating strategy; isolated cells were first separated using Ly6C/MHCII/MerTK/CD64 into 4 GAMM subpopulations and the donor/host purity of each subpopulation was determined using CD45.2 (donor)/CD45.1 (host) expression level. (B) A similar analysis was performed using microglia surface markers Siglec‐H and CX3CR1. CD45⁺ cells were separated using MerTK/CD64/Siglec‐H/CX3CR1. Donor/host purity was then determined using CD45.2 (donor)/CD45.1 (host) expression. This gating strategy showed that only 76.7% of cells in the microglial population were confirmed CD45.1⁺ host cells.