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. 2018 Oct 22;71(1):57–65. doi: 10.1002/iub.1945

Figure 1.

Figure 1

Diagram of AZ‐mediated destruction of target proteins by chimeric proteins ODC and degradation of the KRAS oncoprotein after co‐transfection of RC‐ODC and AZ in human HEK293T cells and the pancreatic cancer cell line PANC‐1. (a) Diagrammatic presentation of the structure of the fusion protein RC‐ODC. The engineered RC‐ODC fusion protein comprises the KRAS‐binding domain RBD + CRD from RAF‐1 fused to the amino terminal and the full‐length ODC at the carboxyl terminus. Homodimers of RC‐ODC are degraded by the 26S proteasome in an AZ stimulation‐dependent manner. (b) RC‐ODC plasmid was co‐transfected with mutant KRAS plasmid and/or AZ plasmid into HEK 293T cells, and the level of the exogenous KRAS protein with the Flag tag was detected 48 h post‐transfection to assess the influence of RC‐ODC and AZ on the KRAS oncoprotein. Ectopic co‐expression of RC‐ODC and AZ notably decreased the KRAS oncoprotein levels (P < 0.05). (c) The level of endogenous KRAS oncoprotein in PANC‐1 cells was detected 48 h post‐transfection to assess the influence of RC‐ODC and AZ on the KRAS oncoprotein. The ectopic co‐expression of RC‐ODC and AZ notably decreased the KRAS oncoprotein levels (P < 0.05).